Datasets:
question stringlengths 1 483 | area stringclasses 22 values | plant_species listlengths 1 3 | options listlengths 3 3 | source stringlengths 12 450 | normalized_plant_species stringclasses 7 values | normalized_area stringclasses 10 values | doi stringlengths 14 40 ⌀ | Year int64 1.99k 2.03k ⌀ | Citations int64 0 1.7k ⌀ | answer int64 0 2 | source_journal stringclasses 119 values | is_expert bool 2 classes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
How many subunits make up the RNA polymerase I complex in Arabidopsis thaliana? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"In Arabidopsis, RNA Pol I consists of 12 protein subunits common to RNA Pol II and Pol III the two others being RNA Pol I-specific subunits.",
"In Arabidopsis, RNA Pol I consists of 14 protein subunits: 12 are RNA Pol I-specific protein subunits and two others are common to RNA Pol II and Pol III.",
"In Arabid... | 10.1093/nar/gkv247 | Model Organisms | GENE REGULATION | 10.1093/nar/gkv247 | 2,015 | 20 | 2 | Nucleic Acids Research | true |
What is in an RNA polymerase I holoenzyme? | GENE REGULATION - TRANSCRIPTION | [
"non-specific"
] | [
"RNA Pol I holoenzyme is the RNA Pol I complex that associates with additional General Transcription Factor (GFTs) to form larger RNA Pol I complexes which are competent for RNA pol I elongation",
"RNA Pol I holoenzyme is the RNA Pol I “core” complex competent to specifically initiate rDNA transcription",
"The ... | 10.1073/pnas.94.22.11869 | Non-specific | GENE REGULATION | 10.1073/pnas.94.22.11869 | 1,997 | 48 | 2 | Proceedings of the National Academy of Sciences | true |
Can the activity of RNA polymerase I complex be regulated by casein kinase 2 (CK2)? | GENE REGULATION - TRANSCRIPTION | [
"non-specific"
] | [
"CK2 interacts with the RNA Pol I holoenzyme and it can phosphorylate transcription factors, thereby increasing rDNA transcription",
"CK2-like protein (CASEIN KINASE2) interacts with the RNA Pol I holoenzyme and can desphosphorylate transcription factors, thereby increasing rDNA transcription.",
"CK2-like prote... | 10.1023/a:1011619413393 | Non-specific | GENE REGULATION | 10.1023/a:1011619413393 | 2,001 | 16 | 0 | Plant Molecular Biology | true |
Which genes are transcribed by RNA polymerase I in Arabidopsis plants? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"The RNA Pol I transcribes a single 45S rDNA copy gene encoding the rRNA 18S, 5.8S and 25S.",
"The RNA Pol I transcribes the rRNA 18S, 5.8S, 5S and 25S ",
"The RNA Pol I transcribes the tandemly organised 45S rDNA encoding the rRNA 18S, 5.8S and 25S. "
] | 10.1105/tpc.18.00874 | Model Organisms | GENE REGULATION | 10.1105/tpc.18.00874 | 2,019 | 124 | 2 | The Plant Cell | true |
Where does RNA polymerase I transcriptional activity take place? | GENE REGULATION - TRANSCRIPTION | [
"non-specific"
] | [
"The RNA Pol I transcription takes place in the nucleolus and more precisely in the dense fibrillary centres (DFC).",
"The RNA Pol I transcription takes place in the nucleolus and more precisely in the dense fibrillary component (DFC).",
"The RNA Pol I transcription takes place in the nucleoplasm and more preci... | 10.1038/s41580-020-0272-6 | Non-specific | GENE REGULATION | 10.1038/s41580-020-0272-6 | 2,020 | 708 | 1 | Nature Reviews Molecular Cell Biology | true |
Can plant microRNA (miRNA) precursors be shortened to express artificial microRNAs (amiRNAs) from viral vectors? | PLANT BIOTECHNOLOGY | [
"non-specific"
] | [
"Yes, plant miRNA precursors can be shortened to express amiRNAs from viral vectors, as such minimal precursors retain the essential structural features necessary for accurate processing and function.",
"No, plant miRNA precursors cannot be shortened to express amiRNAs from viral vectors, as such minimal precurso... | https://doi.org/10.1093/nar/gkad747 | Non-specific | PLANT BIOTECHNOLOGY | 10.1093/nar/gkad747 | 2,023 | 16 | 0 | Nucleic Acids Research | true |
Can TAS1c-based synthetic trans acting small interfering RNAs (syn-tasiRNAs) move throughout Nicotiana benthamiana and induce the systemic silencing of the SULPHUR gene? | PLANT BIOTECHNOLOGY | [
"non-specific"
] | [
"No, TAS1c-based syn-tasiRNAs can move throughout Nicotiana benthamiana, but they are unable to induce systemic silencing of the SULPHUR gene because their movement is restricted to the local tissue where they are produced and do not enter the phloem for long-distance movement.",
"Yes, TAS1c-based syn-tasiRNAs ca... | https://onlinelibrary.wiley.com/doi/10.1111/tpj.15730 | Non-specific | PLANT BIOTECHNOLOGY | 10.1111/tpj.15730 | 2,022 | 10 | 1 | The Plant Journal | true |
Under which molecular form do artificial microRNAs (amiRNAs) and synthetic trans acting small interfering RNAs (syn-tasiRNAs) move throughout Nicotiana benthamiana to induce systemic silencing of endogenous genes? | PLANT BIOTECHNOLOGY | [
"Nicotiana benthamiana"
] | [
"AmiRNAs and syn-tasiRNAs move throughout the plant to induce systemic silencing, typically in the form of ARGONAUTE 1-small RNA complexes that are ready for target-specific silencing.",
"AmiRNAs and syn-tasiRNAs move throughout the plant to induce systemic silencing, typically in the form of small RNA precursors... | https://onlinelibrary.wiley.com/doi/10.1111/tpj.15730 | Solanaceae & Relatives | PLANT BIOTECHNOLOGY | 10.1111/tpj.15730 | 2,022 | 10 | 2 | The Plant Journal | true |
Does multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs (syn-tasiRNAs) enhance plant antiviral resistance? | PLANT BIOTECHNOLOGY | [
"non-specific"
] | [
"Yes, multi-targeting of viral RNAs with syn-tasiRNAs enhances plant antiviral resistance. By expressing multiple syn-tasiRNAs from a single precursor, plants can simultaneously target several regions of a viral genome or even multiple viral genomes, minimizing the likelihood of viral escape mutants, as the virus w... | https://onlinelibrary.wiley.com/doi/10.1111/tpj.14466 | Non-specific | PLANT BIOTECHNOLOGY | 10.1111/tpj.14466 | 2,019 | 44 | 0 | The Plant Journal | true |
How is it possible to fine-tune control target RNAi efficacy in Arabidopsis thaliana using syn-tasiRNAs? | PLANT BIOTECHNOLOGY | [
"Arabidopsis thaliana"
] | [
"It is possible to fine-tune target gene expression with syn-tasiRNAs in Arabidopsis thaliana through two strategies: i) by modulating the level of accumulation of a syn-tasiRNA if changing its precursor position, and ii) by modifying the degree of base-pairing between the 3' end of the syn-tasiRNA and the 5' end o... | https://doi.org/10.1093/nar/gkaa343 | Model Organisms | PLANT BIOTECHNOLOGY | 10.1093/nar/gkaa343 | 2,020 | 20 | 0 | Nucleic Acids Research | true |
Do you find rna polymerase ii transcription start sites only in annotated gene promoter regions? | GENE REGULATION - TRANSCRIPTION | [
"non-specific"
] | [
"No, there are two places where rna polymerase ii can start transcription. 1.) in gene promoters, 2.) in some regions between annotated genes. ",
"Yes, the only location where transcription starts are gene promoters. ",
"No, it is possible to detect transcription start sites in additional regions without annota... | 10.1371/journal.pgen.1007969 | Non-specific | GENE REGULATION | 10.1371/journal.pgen.1007969 | 2,019 | 72 | 2 | PLOS Genetics | true |
Are plant genes are only transcribed in one direction? | GENE REGULATION - TRANSCRIPTION | [
"non-specific"
] | [
"Yes, the only way plant genes can be transcribed is in the direction that results in sense mRNA. ",
"No, but the only type of antisense transcription from genes starts in the 3´-UTR of plant genes. ",
"No, antisense transcription is common. It can either be antisense transcription initiating in the 3´-UTR, or ... | 10.1093/nar/gkz1189 | Non-specific | GENE REGULATION | 10.1093/nar/gkz1189 | 2,019 | 91 | 2 | Nucleic Acids Research | true |
When transcription starts in gene promoters, are only possible outcomes the production of mRNA isoforms? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"No, rna polymerase ii can continue transcription of a different chromosome due to the 3-dimensional genome architecture of plants. ",
"Yes, once rna polymerase ii initiates transcription from a plant gene promoter the only outcome is the production of mRNA isoforms. ",
"No, initiation of rna polymerase ii tran... | 10.1038/s41467-020-16390-7 | Model Organisms | GENE REGULATION | 10.1038/s41467-020-16390-7 | 2,020 | 49 | 2 | Nature Communications | true |
Are there methylation signatures of histone H3 tails that correlate positively with rna polymerase ii transcriptional activity? | GENE REGULATION - POST-TRANSLATIONAL MODIFICATIONS | [
"non-specific"
] | [
"Yes, in particular tri-methylation and di-methylation of histone 3 lysine 4, and histone 3 lysine 36. ",
"Yes, histone 3 lysine 27 tri-methylation.",
"No, only acetylation of histone H3 tails correlates positively with rna polymerase ii transcription. "
] | 10.1016/j.tplants.2020.03.005 | Non-specific | GENE REGULATION | 10.1016/j.tplants.2020.03.005 | 2,020 | 33 | 0 | Trends in Plant Science | true |
Is the transcript resulting from the torpedo-mechanism of transcriptional termination is incorporated into standard genome annotations? | GENE REGULATION - TRANSCRIPTION | [
"non-specific"
] | [
"No, standard methods such as RNA-seq usually rely on 3´- poly-adenylated and 5´-m7G capped RNA species. The transcript associated with torpedo termination lacks both of these features and is therefore usually missing in genome annotations unless the information from suitable methods is used. ",
"No, the reason ... | 10.1186/s12859-021-04259-5. | Non-specific | GENE REGULATION | 10.1186/s12859-021-04259-5 | 2,021 | 1 | 0 | BMC Bioinformatics | true |
How does the UV-B photoreceptor UVR8 regulate gene expression changes in Arabidopsis thaliana plants? | ENVIRONMENT - LIGHT AND TEMPERATURE | [
"Arabidopsis thaliana"
] | [
"After UV-B absorption, the homodimeric UVR8 induces its monomerization, and monomeric UVR8 interacts with the E3 ubiquitin ligases RUP1 and RUP2, leading to gene expression changes. UVR8 is then inactivated through redimerization, facilitated by COP1.",
"After UV-B absorption, the monomeric UVR8 induces its dime... | https://doi.org/10.1073/pnas.2017284118 | Model Organisms | ENVIRONMENT | 10.1073/pnas.2017284118 | 2,021 | 34 | 2 | Proceedings of the National Academy of Sciences | true |
Which are the similitudes and differences between palisade and other photosynthetic cells from Arabidopsis leaves exposed to UV radiation? | ENVIRONMENT - LIGHT AND TEMPERATURE | [
"Arabidopsis thaliana"
] | [
"Palisade cells have a unique morphology, but are transcriptionally similar to other photosynthetic cell types. However, some genes in the phenylpropanoid biosynthesis pathway, which are required for production of the ultraviolet protectant sinapoylmalate, have palisade-enriched expression. ",
"Palisade cells hav... | doi: 10.1093/plcell/koac167. | Model Organisms | ENVIRONMENT | 10.1093/plcell/koac167 | 2,022 | 60 | 0 | The Plant Cell | true |
Which proteins physically interact in the regulation of UV-B tolerance depending on the jasmonic acid signaling pathway in A. thaliana? | ENVIRONMENT - LIGHT AND TEMPERATURE | [
"Arabidopsis thaliana"
] | [
"UV RESISTANCE LOCUS 8 (UVR8), TEOSINTE BRANCHED1, Cycloidea and PCF 4 (TCP4) and LIPOXYGENASE2 (LOX2) physically interacts in the nuclei to increase the DNA binding activity of TCP4 and upregulate the JA biosynthesis.",
"UV RESISTANCE LOCUS 8 (UVR8) and TEOSINTE BRANCHED1, Cycloidea and PCF 4 (TCP4) physically i... | https://doi.org/10.1111/jipb.13648 | Model Organisms | ENVIRONMENT | 10.1111/jipb.13648 | 2,024 | 9 | 1 | Journal of Integrative Plant Biology | true |
Which plant photoreceptors participate in the induction of FERULIC ACID 5-HYDROXYLASE 1 (FAH1), leading to the accumulation of UV-absorbing sinapate esters in Arabidopsis? | ENVIRONMENT - LIGHT AND TEMPERATURE | [
"Arabidopsis thaliana"
] | [
"Both UV RESISTANCE LOCUS 8 (UVR8) UV-B and phytochrome red, but not cryptochrome blue-light photoreceptors, converge on the induction of FERULIC ACID 5-HYDROXYLASE 1 (FAH1), which encodes a key enzyme in the phenylpropanoid biosynthesis pathway. This induction leads to the accumulation of UV-absorbing sinapate est... | https://doi.org/10.1093/plphys/kiae352 | Model Organisms | ENVIRONMENT | 10.1093/plphys/kiae352 | 2,024 | 1 | 2 | Plant Physiology | true |
What is the role of the Jumonji27 (JMJ27) protein during the UV-induced DNA damage in Arabidopsis thaliana plants? | ENVIRONMENT - LIGHT AND TEMPERATURE | [
"Arabidopsis thaliana"
] | [
"JMJ27 is responsible for the UV-induced reduction of H3K9me2 content at chromocenters. In addition, JMJ27 forms a complex with the photodamage recognition factor, DNA Damage Binding protein 2 (DDB2). The fine tuning of H3K9me2 contents orchestrates DDB2 dynamics on chromatin in response to UV-C exposure.",
"JMJ2... | https://doi.org/10.1038/s41477-024-01814-9 | Model Organisms | ENVIRONMENT | 10.1038/s41477-024-01814-9 | 2,024 | 1 | 0 | Nature Plants | true |
Which proteins have been identified as physical interactors of the C subunit of the Nuclear Factor Y 1 (NF-YC1) from common bean (Phaseolus vulgaris)? | ENVIRONMENT - PLANT-SYMBIONTS | [
"Phaseolus vulgaris"
] | [
"SIN1 and NF-YA1 have been identified by bimolecular fluorescence complementation using an NF-YC1-gfp fusion. Both proteins are involved in nodule development. ",
"SIN1 and NIPK have been identified by a yeast two hybrid screening using NF-YC1 as a bait. Both proteins are involved in nodule development. ",
"SIN... | 10.3389/fpls.2022.992543 10.1104/pp.113.230896 | Legumes | ENVIRONMENT | 10.1104/pp.113.230896 | 2,014 | 53 | 1 | Plant Physiology | true |
The promotor of which gene is recognized by the complex that contains NF-YC1 from common bean (Phaseolus vulgaris) and what is its function? | ENVIRONMENT - PLANT-SYMBIONTS | [
"Phaseolus vulgaris"
] | [
"NF-YC1 binds to the promoter of Aurora, a cyclin protein that controls the cell cycle and is involved in root hair development",
"NF-YC1 binds to the promoter of the cyclin P3;1, a cyclin protein that controls the DNA repair and is involved in root hair development",
"NF-YC1 binds to the promoter of the cyclin... | https://doi.org/10.1111/nph.19419 | Legumes | ENVIRONMENT | 10.1111/nph.19419 | 2,023 | 0 | 2 | New Phytologist | true |
How small GTPases from the Rab subfamily have been associated to nodulation in common bean (Phaseolus vulgaris)? | ENVIRONMENT - PLANT-SYMBIONTS | [
"Phaseolus vulgaris"
] | [
"RabA2a from common bean was identified by its differential expression in response to two different strains of Rhizobium leguminosarum. It is expressed in roots, particularly in athrichoblasts and the protein is located in vesicles. Genetic studies have shown that RabA2a is required for root growth, early infection... | 10.1094/MPMI, 10.1105/tpc.108.063420 | Legumes | ENVIRONMENT | 10.1105/tpc.108.063420 | 2,009 | 51 | 1 | The Plant Cell | true |
How the trimer of NF-YC that acts during symbiosis was identified in common bean (Phaseolus vulgaris)? | ENVIRONMENT - PLANT-SYMBIONTS | [
"Phaseolus vulgaris"
] | [
"The subunits of the trimer formed by NF-YA1, NF-YB1 and NF-YC1 were selected according to their expression pattern in root hairs and tested by coimmunoprecipitation assays",
"The subunits of the trimer formed by NF-YA1, NF-YB7 and NF-YC1 were selected according to their expression pattern at early stages of the ... | 10.1104/pp.15.01144 | Legumes | ENVIRONMENT | 10.1104/pp.15.01144 | 2,015 | 25 | 1 | Plant Physiology | true |
How RNAs produced by Bradyrhizobium japonicum can modulate soybean (Glycine max) genes to promote nodulation? | ENVIRONMENT - PLANT-SYMBIONTS | [
"Glycine max"
] | [
"Small fragments of RNA are produced in Bradyrhizobium japonicum by degradation of tRNAs (tRFs) and transported to soybean cells, where postranscriptionally regulate mRNA targets using ARGONAUTE 1 and the host RNAi machinery. tRFs are positive regulators of nodulation since their mRNA targets repress nodule formati... | 10.1126/science.aav8907 | Legumes | ENVIRONMENT | 10.1126/science.aav8907 | 2,019 | 221 | 0 | Science | true |
What is the impact of the presence of an IR inserted near the sunflower HaWRKY6 locus? | GENE REGULATION - PTGS | [
"Helianthus annuus"
] | [
"The presence of an IR inserted near the sunflower HaWRKY6 locus regulates the expression of the HaWRKY6 gene by altering the chromatin structure. The IR is transcribed, and its transcript gives rise to 21-nt siRNAs, which trigger posttranscriptional silencing. This epigenetic mark stabilizes the formation of two a... | https://doi.org/10.1073/pnas.1903131116 | Other Herbaceous Crops, Spices, Fibers & Weeds | GENE REGULATION | 10.1073/pnas.1903131116 | 2,019 | 41 | 1 | Proceedings of the National Academy of Sciences | true |
How can the insertion of transposon-derived inverted repeats (IRs) impact transcription in Arabidopsis thaliana? | GENE REGULATION - PTGS | [
"Arabidopsis thaliana"
] | [
"The presence of transposon-derived inverted repeats (IRs), particularly near coding genes can cause chromatin rearrangements. These rearrangements often lead to the formation of short-range chromatin loops, which are associated with either the activation or repression of transcription in neighboring genes.",
"Th... | https://doi.org/10.1016/j.celrep.2023.112029 | Model Organisms | GENE REGULATION | 10.1016/j.celrep.2023.112029 | 2,023 | 12 | 0 | Cell Reports | true |
How do transposon-derived inverted repeats (IRs) impact the adaptation of Arabidopsis thaliana? | GENE REGULATION - PTGS | [
"Arabidopsis thaliana"
] | [
"The natural variation in the presence of transposon-derived inverted repeats (IRs) among Arabidopsis thaliana accessions and their correlation with variations in gene expression can explain the differential phenotypes observed among these accessions. Therefore, IRs represent powerful elements in adaptive evolution... | https://doi.org/10.1016/j.celrep.2023.112029 | Model Organisms | GENE REGULATION | 10.1016/j.celrep.2023.112029 | 2,023 | 12 | 0 | Cell Reports | true |
Is RdDM-dependent silencing of transposable elements always associated with the transcriptional repression of the TEs and their surrounding regions in Arabidopsis? | GENE REGULATION - PTGS | [
"Arabidopsis thaliana"
] | [
"In the case of transposon-derived inverted repeats (IRs), non-canonical RdDM, triggered by the production of 24-nt siRNAs from the single-stranded secondary structure formed by Pol IV-driven IR transcription, and the resulting DNA condensation, appears to function differently. For example, it can alter chromatin t... | https://doi.org/10.1016/j.celrep.2023.112029 | Model Organisms | GENE REGULATION | 10.1016/j.celrep.2023.112029 | 2,023 | 12 | 1 | Cell Reports | true |
Which transposable elements have been identified as capable of regulating the expression of the EFR gene in Arabidopsis thaliana? | GENE REGULATION - PTGS | [
"Arabidopsis thaliana"
] | [
"A transposon-derived inverted repeat (IR) element located downstream of the EFR gene in Arabidopsis thaliana, named Ea-IR, has the capacity to regulate EFR gene expression. Depending on its methylation state, the Ea-IR can rearrange chromatin topology, creating a short-range chromatin loop that enhance EFR gene ex... | https://doi.org/10.1101/2023.10.06.561201 | Model Organisms | GENE REGULATION | 10.1101/2023.10.06.561201 | 2,023 | 0 | 0 | null | true |
What are the two main molecular mechanisms for the co-regulation of hypocotyl growth by auxin and gibberellin (GA) in Arabidopsis? | HORMONES | [
"Arabidopsis thaliana"
] | [
"The two primary mechanisms are: (1) Auxin-induced gibberellin catabolism: Auxin stimulates the ARF-dependent transcriptional upregulation of genes encoding GA 2-oxidases, which are key enzymes in gibberellin inactivation. This leads to decreased local GA production in hypocotyl tissues, driving cell elongation. (2... | https://doi.org/10.1104/pp.106.084871 https://doi.org/10.7554/eLife.03031 | Model Organisms | HORMONES | 10.7554/eLife.03031 | 2,014 | 459 | 2 | eLife | true |
What three molecular changes explain the conversion of an ancestral carboxylesterase into a gibberellin receptor? | HORMONES | [
"non-specific"
] | [
"The conversion of an ancestral carboxylesterase into a gibberellin (GA) receptor is explained by the following three molecular changes: (1) Loss of enzymatic activity: The carboxylesterase's enzymatic function was reduced or eliminated, permitting the protein to specialize as a receptor. This loss enabled the focu... | DOI: 10.1073/pnas.1806040115 | Non-specific | HORMONES | 10.1073/pnas.1806040115 | 2,018 | 56 | 2 | Proceedings of the National Academy of Sciences | true |
What is the impact of gibberellins in the control of flowering time in Arabidopsis and what are the main flowering-time genes involved? | HORMONES | [
"Arabidopsis thaliana"
] | [
"Gibberellins are essential to repress flowering under non-inductive short-day conditions. They do it by decreasing the expression of key flowering-time genes, such as LEAFY and SUPPRESSOR OF CONSTANS OVEREXPRESSION (SOC1). ",
"Gibberellins are essential to promote flowering under non-inductive short-day conditio... | DOI: 10.1105/tpc.10.5.791. doi: 10.1046/j.1365-313x.2003.01833.x | Model Organisms | HORMONES | 10.1046/j.1365-313x.2003.01833.x | 2,003 | 463 | 1 | The Plant Journal | true |
What molecular mechanisms regulate DELLA activity in Arabidopsis, beyond the control of cellular DELLA levels? | HORMONES | [
"Arabidopsis thaliana"
] | [
"DELLA activity, defined by the interaction with transcription factors and other transcriptional regulators, is regulated by postranslational modifications that alter DELLA’s capacity to establish these interactions. Among them, three are the best studied ones: In Arabidopsis, O-fucosylation catalyzed by SPINDLY (S... | doi: 10.1101/gad.270587.115. doi: 10.1038/nchembio.2320. doi: 10.1007/s00425-024-04565-1 | Model Organisms | HORMONES | 10.1007/s00425-024-04565-1 | 2,024 | 0 | 0 | Planta | true |
What is the experimental evidence for the involvement of NPF3 in gibberellin-dependent regulation of Arabidopsis root growth? | HORMONES | [
"Arabidopsis thaliana"
] | [
"NPF3 is a gibberellin influx carrier expressed in the root endodermis. Gibberellin accumulation in the endodermis drives cell expansion in this cell type, from which growth of the whole organ is coordinated. Overexpression of NPF3 causes a phenotype associated to gibberellin hypersensitivity in roots.",
"NPF3 is... | doi: 10.1038/ncomms11486 | Model Organisms | HORMONES | 10.1038/ncomms11486 | 2,016 | 181 | 0 | Nature Communications | true |
Does the alfalfa dwarf cytorebdovirus P protein exhibit activity as a suppressor of local and/or systemic RNA silencing? | ENVIRONMENT - BIOTIC STRESS | [
"non-specific"
] | [
" The phosphoprotein (P) encoded by alfalfa dwarf virus (ADV) is a suppressor of RNA silencing. ADV P has a very strong local suppressor activity and prevents RNAi accumulation, but weakly suppresses systemic RNA silencing. Protein-protein interaction assays determined that the suppression mechanism appears to invo... | http://dx.doi.org/10.1016/j.virusres.2016.08.008 | Non-specific | ENVIRONMENT | 10.1016/j.virusres.2016.08.008 | 2,016 | 16 | 2 | Virus Research | true |
Is the transmission of CMV through seeds possible in pepper, and if so, what is the estimated rate of such transmission? | ENVIRONMENT - BIOTIC STRESS | [
"Capsicum annuum"
] | [
" When CMV seed growth tests were performed in pepper pots, no transmission was detected in either the seed coat or the embryo. The final transmission rate of the seeds was 0%.",
" When CMV seed growth tests were performed in pots on peppers, transmission was detected only in seed coat. CMV infection in seeds ran... | doi:10.1016/j.jviromet.2009.09.026 | Solanaceae & Relatives | ENVIRONMENT | 10.1016/j.jviromet.2009.09.026 | 2,010 | 60 | 2 | Journal of Virological Methods | true |
What is the relationship between the efficiency of PVY inhibition across multiple strains (PVYN, PVYO, and PVYNTN) and the expression of Cas13 with specific gRNA cassettes in transgenic potato lines? | ENVIRONMENT - BIOTIC STRESS | [
"Solanum tuberosum"
] | [
"Efficiency of PVY inhibition against multiple strains of PVYN, PVYO, and PVYNTN does not correlate with Cas13 with specific gRNA cassette expression in transgenic potato lines.",
" Efficiency of PVY inhibition against multiple strains of PVYN, PVYO, and PVYNTN positively correlated with Cas13 with specific gRNA... | https://doi.org/10.1080/21645698.2022.2080481 | Solanaceae & Relatives | ENVIRONMENT | 10.1080/21645698.2022.2080481 | 2,022 | 13 | 1 | GM Crops & Food | true |
What is the effect of silencing the stress-induced gene encoding Kunitz peptidase inhibitor-like protein on the death rate of TMV-infected Nicotiana benthamiana plants ? | ENVIRONMENT - BIOTIC STRESS | [
"Nicotiana benthamiana"
] | [
"Compared to both the control group and plants with elevated KPILP levels in Nicotiana benthamiana, silencing the stress-induced gene encoding Kunitz peptidase inhibitor-like protein (KPILP) increases the death rate of TMV-infected plants. Systemic infection of N. benthamiana plants with tobacco mosaic virus (TMV) ... | https://doi.org/10.3389/fpls.2023.1224958 | Solanaceae & Relatives | ENVIRONMENT | 10.3389/fpls.2023.1224958 | 2,023 | 5 | 1 | Frontiers in Plant Science | true |
Considering emerging studies, what are the critical factors contributing to the manifestation of virus-induced symptoms in plants through the manipulation of plant cellular processes by viruses? | ENVIRONMENT - BIOTIC STRESS | [
"non-specific"
] | [
" Key factors involved in the mechanisms by which viruses manipulate plant cellular processes inducing symptoms have been identified as hormonal manipulation and gene expression changes.",
"Key factors involved in the mechanisms by which viruses manipulate plant cellular processes inducing symptoms have been ide... | https://doi.org/10.3390/plants12152830 | Non-specific | ENVIRONMENT | 10.3390/plants12152830 | 2,023 | 26 | 2 | Plants | true |
Which gene was targeted using CRISPR/Cas9 to delay flowering time in Medicago sativa (alfalfa)? | PLANT BIOTECHNOLOGY | [
"Medicago sativa"
] | [
"The polyester synthase-like gene At1g73750 has been disrupted using CRISPR/Cas9 to delay flowering time in Medicago sativa.",
"The FT1 gene has been disrupted using CRISPR/Cas9 to delay flowering time in Medicago sativa.",
"The polyester synthase-like gene MSAD_264347 has been disrupted using CRISPR/Cas9 to de... | https://doi.org/10.1007/s00299-023-02997-9 | Legumes | PLANT BIOTECHNOLOGY | 10.1007/s00299-023-02997-9 | 2,023 | 2 | 2 | Plant Cell Reports | true |
What is one of the main challenges in applying CRISPR/Cas9 technology to crop genomes? | PLANT BIOTECHNOLOGY | [
"non-specific"
] | [
"Efficient delivery of CRISPR/Cas9 components into plant cells and successful regeneration of edited plants.",
"CRISPR/Cas9 cannot target coding regions of plant genomes due to polyploidy.",
"CRISPR/Cas9 requires the presence of specific RNA polymerases unique to animals, making it less efficient in plants."
] | https://doi.org/10.1016/j.plantsci.2023.111809 | Non-specific | PLANT BIOTECHNOLOGY | 10.1016/j.plantsci.2023.111809 | 2,023 | 0 | 0 | Plant Science | true |
How does the Protospacer Adjacent Motif (PAM) influence the CRISPR/Cas9 genome editing process? | GENOME AND GENOMICS | [
"non-specific"
] | [
"The PAM sequence determines the efficiency of DNA repair after Cas9 introduces a double-strand break.",
"The PAM sequence is incorporated into the guide RNA to enhance its stability during the editing process.",
"The PAM sequence is required for Cas9 to identify and bind the target DNA site, enabling precise c... | https://doi.org/10.1093/aob/mcae191 | Non-specific | GENOME AND GENOMICS | 10.1093/aob/mcae191 | 2,024 | 0 | 2 | Annals of Botany | true |
What is the impact of disrupting the SPL13 gene in lettuce via genome editing, and why could this strategy be useful for developing a commercial variety? | PLANT BIOTECHNOLOGY | [
"Lactuca sativa"
] | [
"Disrupting the SPL13 gene in lettuce via genome editing delays flowering, increases biomass, and enhances leaf production. This strategy is useful for developing a commercial variety as it extends the vegetative growth phase, resulting in higher yields and improved agronomic traits that are desirable for market pr... | https://doi.org/10.1007/s00299-022-02952-0 | Other Herbaceous Crops, Spices, Fibers & Weeds | PLANT BIOTECHNOLOGY | 10.1007/s00299-022-02952-0 | 2,022 | 6 | 0 | Plant Cell Reports | true |
Which genes were successfully modified using cytosine base editing (CBE) in alfalfa to confer herbicide tolerance, and why was a dead Cas9 (dCas9) used in this process? | PLANT BIOTECHNOLOGY | [
"Medicago sativa"
] | [
"The ALSI and ALSII genes were successfully modified using CBE to confer herbicide tolerance. A dead Cas9 (dCas9), fused to a cytosine deaminase, was used to cleave the DNA at specific sites, enabling the integration of a resistance gene into the target locus.",
"The ALS1 and ALS2 genes were successfully modified... | https://doi.org/10.1007/s00299-021-02827-w | Legumes | PLANT BIOTECHNOLOGY | 10.1007/s00299-021-02827-w | 2,022 | 15 | 1 | Plant Cell Reports | true |
Which is the master regulator gene involved in branching regulation in Arabidopsis thaliana? And how is it expression regulated? | GROWTH AND DEVELOPMENT | [
"Arabidopsis thaliana"
] | [
"In Arabidopsis thaliana, the master regulator of branching is BRANCHED1 (BRC1), a transcription factor from the TCP class II family. This gene is regulated by numerous environmental and endogenous conditions. Regarding environmental conditions, being shaded by neighbor plants or increasing far red light promote BR... | doi: 10.3389/fpls.2014.00741 | Model Organisms | GROWTH AND DEVELOPMENT | 10.3389/fpls.2014.00741 | 2,015 | 222 | 0 | Frontiers in Plant Science | true |
How is the long noncoding RNA APOLO involved in the regulation of branching in Arabidopsis thaliana? | GROWTH AND DEVELOPMENT | [
"Arabidopsis thaliana"
] | [
"The long non coding RNA APOLO participates in branching regulation through the epigenetic modulation of BRC1expression. This regulation occurs in the context of shade or under high levels of far red light. Different levels of APOLO modulate a chromatin loop opening or formation. This chromatin loop encompasses BRC... | DOI 10.15252/embj.2023113941 | Model Organisms | GROWTH AND DEVELOPMENT | 10.15252/embj.2023113941 | 2,023 | 3 | 0 | The EMBO Journal | true |
In which context does the long noncoding RNA APOLO participate in BRC1 expression modulation in Arabidopsis thaliana? | GROWTH AND DEVELOPMENT | [
"Arabidopsis thaliana"
] | [
"The long non coding RNA APOLO modulates BRC1 expression in the context of shade or under high far red light levels.",
"The long non coding RNA APOLO modulates BRC1 expression in the context of low far red levels.",
"The long non coding RNA APOLO modulates BRC1 expression in the context of high temperatures"
] | DOI 10.15252/embj.2023113941 | Model Organisms | GROWTH AND DEVELOPMENT | 10.15252/embj.2023113941 | 2,023 | 3 | 0 | The EMBO Journal | true |
Which are the most important responses of Arabidopsis thaliana plants to the shade avoidance syndrome? | ENVIRONMENT - LIGHT AND TEMPERATURE | [
"Arabidopsis thaliana"
] | [
"In Arabidopsis thaliana, shade light signals delay flowering and promote branching. ",
"In Arabidopsis thaliana, the shade avoidance syndrome induces seeds germination and suppresses hypocotyl and petiole elongation.",
"In Arabidopsis thaliana, the shade avoidance syndrome presents different responses dependin... | doi: 10.1199/tab.0157 | Model Organisms | ENVIRONMENT | 10.1199/tab.0157 | 2,012 | 311 | 2 | The Arabidopsis Book | true |
How is hyponasty regulated in Arabidopsis thaliana? | ENVIRONMENT - LIGHT AND TEMPERATURE | [
"Arabidopsis thaliana"
] | [
"The hyponasty response is the leaf upward movement driven by a higher rate of cell expansion on de abaxial side compared with the adaxial. This difference in cell expansion depends on auxin biosynthesis, transport and distribution, which is regulated by R/FR light. Low R/FR inactivates phyB, allowing PIFs to accum... | DOI 10.15252/embj.2023113941 | Model Organisms | ENVIRONMENT | 10.15252/embj.2023113941 | 2,023 | 3 | 0 | The EMBO Journal | true |
Which genes (AGI) encode structural proteins of cytochrome c oxidase in Arabidopsis thaliana? Mention whether there is homology with any yeast and/or mammalian genes? | PHYSIOLOGY AND METABOLISM | [
"Arabidopsis thaliana"
] | [
"Cytochrome c Oxidase is composed of several subunits in Arabidopsis thaliana. The enzymatic core is highly conserved between plants, mammals and yeasts and the genes are normally encoded by the mitochondrial genome. The Arabidopsis genes are COX1 (ATMG01360), COX2 (ATMG00160) and COX3 (ATMG01520). The rest of the ... | https://doi.org/10.3390/ijms19030662 | Model Organisms | PHYSIOLOGY AND METABOLISM | 10.3390/ijms19030662 | 2,018 | 97 | 2 | International Journal of Molecular Sciences | true |
Describe the main phenotypes of the Cytochrome c mutants in Arabidopsis thaliana and the associated molecular pathways that are affected | PHYSIOLOGY AND METABOLISM | [
"Arabidopsis thaliana"
] | [
" Arabidopsis thaliana has 2 genes that encode for the heme protein Cytochrome c, CYTC-1 and CYTC-2. This protein is involved in the electron transfer between complex III and complex IV of the mitochondrial electron transport chain. The mutation in the CYTC genes in Arabidopsis generates different phenotypes depend... | https://doi.org/10.1111/tpj.13845, https://doi.org/10.1111/nph.18287, https://doi.org/10.1111/nph.19506, | Model Organisms | PHYSIOLOGY AND METABOLISM | 10.1111/nph.19506 | 2,024 | 6 | 2 | New Phytologist | true |
Does the Arabidopsis cytochrome CYT-C2 gene have any function that is not redundant with the CYT-C1 gene? If there is any molecular mechanism described, please explain it. | PHYSIOLOGY AND METABOLISM | [
"Arabidopsis thaliana"
] | [
"Both CYT-C genes present redundancy in most biological processes. This is because both are the result of a recent duplication. However, beyond the fact that at the protein sequence level they are practically identical, their promoters have different expression patterns. During germination, a repression of the CYT-... | https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18287 | Model Organisms | PHYSIOLOGY AND METABOLISM | 10.1111/nph.18287 | 2,022 | 9 | 1 | New Phytologist | true |
In the aerial tissue of plant organisms, both chloroplasts and mitochondria have the capacity to synthesize ATP. In Arabidopsis, do cytoplasmic and nuclear metabolism directly use ATP from both sources equally? | PHYSIOLOGY AND METABOLISM | [
"Arabidopsis thaliana"
] | [
"Currently, very few studies have delved into this topic. However, the use of fluorescent ATP sensors has allowed the evaluation of the level of ATP in the cytoplasm of different Arabidopsis tissues, under light and dark conditions. In addition, the evolution of ATP was evaluated by inhibiting the mitochondrial ele... | https://doi.org/10.7554/eLife.26770 | Model Organisms | PHYSIOLOGY AND METABOLISM | 10.7554/eLife.26770 | 2,017 | 130 | 2 | eLife | true |
The COX10 and COX15 genes are involved in the biosynthesis of the heme a group, which is essential for the assembly of cytochrome c oxidase (CcO) in humans and yeasts. Are there homologs of these genes in Arabidopsis? Is their function conserved? Is there a molecular mechanism that describes its participation in the biosynthesis of the heme a and assembly of CcO in Arabidopsis? | PHYSIOLOGY AND METABOLISM | [
"Arabidopsis thaliana"
] | [
"Both genes have homologs encoded in the Arabidopsis genome. COX10 is encoded in the locus AT2G44520, while COX15 is encoded in AT5G56090. The function of both genes in the assembly of CcO would be conserved, since these Arabidopsis thaliana genes are able to rescue mitochondrial respiration in mutants of its homol... | https://doi.org/10.1093/jxb/erv381, https://doi.org/10.3390/ijms19030662 | Model Organisms | PHYSIOLOGY AND METABOLISM | 10.3390/ijms19030662 | 2,018 | 97 | 2 | International Journal of Molecular Sciences | true |
How LncRNA MSTRG.13420 formed R-loop functions in cold response of rice? | GENOME AND GENOMICS | [
"Oryza sativa"
] | [
"LncRNA MSTRG.13420 located in downstream region of OsDof16 was transcribed from antisense strand and significantly downregulated in cold treatment. LncRNA MSTRG.13420 formed R-loop to negatively regulate OsDof16, and act as posstive regulator in cold response in rice.",
"LncRNA MSTRG.13420 located in promoter of... | 10.1111/nph.19315. | Model Organisms | GENOME AND GENOMICS | 10.1111/nph.19315 | 2,023 | 2 | 1 | New Phytologist | true |
What’s the relationship between DNA G4 intensity and expression levels of G4-overlapping genes in rice genome? | GENOME AND GENOMICS | [
"Oryza sativa"
] | [
"G4s intensity exhibited a negative association with expression levels of G4-overlapping genes at transcript end sites (TTSs), while G4s in gene bodies was positively corresponded with expression levels.",
"G4s intensity exhibited a positive association with expression levels of G4-overlapping genes at transcript... | doi: 10.1093/plphys/kiab566. | Model Organisms | GENOME AND GENOMICS | 10.1093/plphys/kiab566 | 2,021 | 28 | 2 | Plant Physiology | true |
How 5mC DNA methylation affect i-motif formation under different pH condition in rice genome? | GENOME AND GENOMICS | [
"Oryza sativa"
] | [
"Subset of i-motif folded at pH 5.5 show high methylation levels than those folded at pH 7.0, and core regions of i-motifs trend to be higher methylated than flank regions.",
"Subset of i-motif folded at pH 7.0 show high methylation levels than those folded at pH 5.5, and flank regions of i-motifs trend to be hig... | 10.1093/nar/gkad1245. | Model Organisms | GENOME AND GENOMICS | 10.1093/nar/gkad1245 | 2,024 | 8 | 2 | Nucleic Acids Research | true |
In which genomic regions the i-motif structure trend to be present or depleted? | GENOME AND GENOMICS | [
"Oryza sativa"
] | [
"The rice genome was divided into seven genomic subregions, including 5′UTRs, 3′UTRs, exons, introns, downstream and distal intergenic regions. The i-motif structure trend to present in promoters and 5′UTRs, and depleted in exons and distal intergenic regions.",
"The rice genome was divided into seven genomic sub... | 10.1093/nar/gkac121. | Model Organisms | GENOME AND GENOMICS | 10.1093/nar/gkac121 | 2,022 | 34 | 0 | Nucleic Acids Research | true |
What are the intrinsic sequence feature for Cold-induced R-loop regions. | GENOME AND GENOMICS | [
"Oryza sativa"
] | [
"Cold-induced R-loops have highest GC content compare to other genomic regions, GCGGC and CCTCC binding motifs of C2H2 family were enriched in Cold-induced R-loops, which were reported to be involved in plant stress response.",
"Cold-induced R-loops have highest GC content compare to other genomic regions, CCGCC ... | 10.1111/nph.19315. | Model Organisms | GENOME AND GENOMICS | 10.1111/nph.19315 | 2,023 | 2 | 1 | New Phytologist | true |
In what ways has the functional characterization of BBX proteins in crop species, such as BBX32 in soybean, BBX21 in potato, or BBX24 in Chrysanthemum, advanced our understanding of their potential applications in improving agronomic traits? | PLANT BIOTECHNOLOGY | [
"Solanum tuberosum",
"Glycine max",
"Chrysanthemum"
] | [
"Functional characterization of BBX proteins indicates they only affect flowering time and have no relevance to traits like photosynthesis or yield improvement.",
"The studies have shown that BBX proteins are primarily involved in promoting vegetative growth, with no significant impact on yield or stress toleranc... | https://doi.org/10.1104/pp.17.01417 | Other Herbaceous Crops, Spices, Fibers & Weeds | PLANT BIOTECHNOLOGY | 10.1104/pp.17.01417 | 2,018 | 31 | 2 | Plant Physiology | true |
How does BBX24 influence the interaction between DELLAs and PIF4 in the context of shade avoidance? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"BBX24 has no effect on the interaction between DELLAs and PIF4, regardless of light conditions.",
"BBX24 promotes the interaction between DELLAs and PIF4, leading to reduced growth in shaded environments.",
"BBX24 sequesters DELLAs, preventing them from inhibiting PIF4, which enhances cell elongation and growt... | https://doi.org/10.1038/ncomms7202 | Model Organisms | GENE REGULATION | 10.1038/ncomms7202 | 2,015 | 89 | 2 | Nature Communications | true |
How does UV-B radiation impact Arabidopsis BBX29 expression and its role in plant defense? | ENVIRONMENT - BIOTIC STRESS | [
"Arabidopsis thaliana"
] | [
"UV-B radiation strongly induces AtBBX29 expression in a UVR8-dependent manner, enhancing the accumulation of phenolic compounds that boost plant resistance to pathogens.",
"UV-B radiation decreases AtBBX29 expression, leading to reduced accumulation of phenolic compounds and increased susceptibility to pathogens... | https://link.springer.com/article/10.1007/s43630-023-00391-8 | Model Organisms | ENVIRONMENT | 10.1007/s43630-023-00391-8 | 2,023 | 6 | 0 | Photochemical & Photobiological Sciences | true |
In what ways do photoreceptors, such as phytochromes and cryptochromes, interact with light-regulated transcription factors like HY5, BBXs and PIFs to regulate the developmental processes in crops, and how can these interactions be targeted for crop improvement? | GROWTH AND DEVELOPMENT | [
"non-specific"
] | [
"Interactions between photoreceptors and light-regulated transcription factors like HY5, BBXs and PIFs play a crucial role in regulating key developmental processes, such as flowering time and leaf expansion, and can be targeted in breeding programs to enhance crop performance.",
"Photoreceptors and light-regulat... | https://doi.org/10.1104/pp.17.01417 | Non-specific | GROWTH AND DEVELOPMENT | 10.1104/pp.17.01417 | 2,018 | 31 | 0 | Plant Physiology | true |
How do ABA sensitivity and stomatal responses differ between BBX21-OE lines and wild-type potato plants? | HORMONES | [
"Solanum tuberosum"
] | [
"BBX21-OE lines exhibit increased sensitivity to ABA, resulting in reduced stomatal openings compared to wild-type plants when both are exposed to ABA.",
"BBX21-OE lines show improved tolerance to ABA, maintaining stomatal openings under lower ABA concentrations, whereas wild-type plants exhibit reduced stomatal ... | https://doi.org/10.1111/tpj.15499 | Solanaceae & Relatives | HORMONES | 10.1111/tpj.15499 | 2,021 | 18 | 1 | The Plant Journal | true |
What role does the trans-acting small interfering RNA (tasiRNA) pathway play in lateral root development in Arabidopsis thaliana and what are the components involved? | GENE REGULATION - PTGS | [
"Arabidopsis thaliana"
] | [
"In Arabidopsis thaliana, cytokinins, miR390, TAS3, and their ARFs targets define a regulatory network quantitatively controlling lateral root growth. Mutations affecting the abundance of TAS3-derived tasiRNAs lead to inhibition of lateral root growth. miR390 is induced in response to cytokinins during lateral root... | https://doi.org/10.1105/tpc.109.072553 | Model Organisms | GENE REGULATION | 10.1105/tpc.109.072553 | 2,010 | 486 | 2 | The Plant Cell | true |
By which mechanism has the function of the transcription factor LEAFY evolved in land plants? | EVOLUTION | [
"Arabidopsis thaliana",
"Physcomitrella patens"
] | [
"The plant-specific transcription factor LEAFY is found in all land plants, usually as a single copy gene. While it controls general aspects of the life cycle in the basal plant Physcomitrella patens, it has more specialized functions in flowering plants, where it specifically induces floral fate during the reprodu... | https://doi.org/10.1126/science.1108229 https://doi.org/10.1126/science.1248229 | Model Organisms | EVOLUTION | 10.1126/science.1248229 | 2,014 | 121 | 0 | Science | true |
Where is the biogenesis of trans-acting siRNA taking place in plant cells? | GENE REGULATION - PTGS | [
"Arabidopsis thaliana"
] | [
"Trans-acting small interfering RNAs (ta-siRNAs) from the TAS3 precursor are produced by cleavage of the TAS3 precursor by the AGO1/miR173 complex, priming it for conversion into single-stranded RNA by the DICER LIKE 4 (DCL4). AGO7 and DCL4 accumulate in cytoplasmic P-bodies. These foci are membrane-associated si... | https://doi.org/10.1038/emboj.2012.20 | Model Organisms | GENE REGULATION | 10.1038/emboj.2012.20 | 2,012 | 122 | 1 | The EMBO Journal | true |
In Arabidopsis thaliana, what role does the microtubule network play during lateral root initiation and emergence? | CELL BIOLOGY AND CELL SIGNALING | [
"Arabidopsis thaliana"
] | [
"The rearrangement of the microtubule (MT) cytoskeleton, alongside changes in cell-wall properties, contributes to the symmetric radial expansion necessary for endodermis thinning. In the endodermis, the organization and response of the cortical microtubule lattice are polarised. On the inner side, in contact with ... | https://doi.org/10.1016/j.cub.2019.06.039 https://doi.org/10.1126/sciadv.abm4974 | Model Organisms | CELL BIOLOGY AND CELL SIGNALING | 10.1126/sciadv.abm4974 | 2,022 | 28 | 2 | Science Advances | true |
What role does TOR play during lateral root formation in Arabidopsis thaliana, and by which mechanism? | PHYSIOLOGY AND METABOLISM | [
"Arabidopsis thaliana"
] | [
"The Target-of-Rapamycin (TOR) kinase plays a crucial role in lateral root (LR) formation in Arabidopsis thaliana by integrating local auxin signaling with systemic metabolic cues. TOR is specifically repressed in the lateral root domain, where it regulates the transcription of key auxin-induced transcription facto... | https://doi.org/10.15252/embj.2022111273 | Model Organisms | PHYSIOLOGY AND METABOLISM | 10.15252/embj.2022111273 | 2,023 | 27 | 2 | The EMBO Journal | true |
What is ‘state transitions’ and which molecular processes are involved in its regulation in plants? | GROWTH AND DEVELOPMENT | [
"non-specific"
] | [
"State transitions is the process through which plants rebalance the activity of photosystem I (PSI) and photosystem II (PSII) under fluctuating light quality conditions. Two states were defined, one induced by light preferentially absorbed by PSII (State II), and the other induced by light preferentially absorbed ... | https://doi.org/10.1016/j.bbabio.2010.11.005 | Non-specific | GROWTH AND DEVELOPMENT | 10.1016/j.bbabio.2010.11.005 | 2,011 | 225 | 0 | Biochimica et Biophysica Acta (BBA) - Bioenergetics | true |
How do you explain the rise in transient chlorophyll a fluorescence when leaves are exposed to light based on the QA model? | GROWTH AND DEVELOPMENT | [
"non-specific"
] | [
"Based on the QA model, the increase in transient fluorescence yield is related to the reduction of electron acceptors and the relaxation of fluorescence quenchers in the photosynthetic electron transport chain. Upon light absorption, photosystem II (PSII) chlorophyll P680 reduces QA (a PSII-bound quinone). Reduced... | https://doi.org/10.1093/jxb/erad252 | Non-specific | GROWTH AND DEVELOPMENT | 10.1093/jxb/erad252 | 2,023 | 25 | 2 | Journal of Experimental Botany | true |
Which and where reactive oxygen species (ROS) are produced in the chloroplast photosynthetic electron transport chain and what is defined as photoinhibition? | GROWTH AND DEVELOPMENT | [
"non-specific"
] | [
"The main site of ROS production in the photosynthetic electron transport chain are photosystem I and II (PSI and PSII). Over-excitation of the photosystems mainly generate singlet excited oxygen (1O2*) at the level of PSII and superoxide radicals (O2.-) and hydrogen peroxide (H2O2) at PSI. ROS production in the ph... | https://doi.org/10.1042/BST20211246 | Non-specific | GROWTH AND DEVELOPMENT | 10.1042/BST20211246 | 2,022 | 24 | 0 | Biochemical Society Transactions | true |
What is the role of the transporters TPT and PHT2;1 in regulating chloroplastic phosphorus (Pi) homeostasis and photosynthesis in Arabidopsis thaliana? | GROWTH AND DEVELOPMENT | [
"Arabidopsis thaliana"
] | [
"TPT and PHT2;1 are molecular transporters that import phosphorus (Pi) to the chloroplast stroma in Arabidopsis thaliana. While TPT couples Pi import with triose phosphate export (produced by photosynthesis) to the cytosol, PHT2;1 imports both Pi and H+/Na+. Although both transporters contribute to stromal Pi homeo... | https://doi.org/10.1093/plphys/kiae241 | Model Organisms | GROWTH AND DEVELOPMENT | 10.1093/plphys/kiae241 | 2,024 | 4 | 0 | Plant Physiology | true |
How can the activity of the plastid terminal oxidase (PTOX) be explained by the PSII proximity hypothesis in plants? | GROWTH AND DEVELOPMENT | [
"non-specific"
] | [
"Photosystem II (PSII) and PTOX are located in different sub-compartments of the chloroplast. While PTOX is specifically targeted to the grana, PSII is localized in the stromal lamellae. Since PTOX oxidizes plastoquinol and reduces O2 to produce H2O, its activity will depend on the availability of its substrates. T... | https://doi.org/10.1038/s41467-023-44454-x | Non-specific | GROWTH AND DEVELOPMENT | 10.1038/s41467-023-44454-x | 2,024 | 6 | 1 | Nature Communications | true |
What is the magnitude of alternative splicing regulation in rice leaves undergoing heat stress? | GENE REGULATION - ALTERNATIVE SPLICING | [
"Oryza sativa"
] | [
"Alternative splicing is widespread in rice leaves undergoing heat stress. For instance, genes coding for key regulators of gene expression can be mispliced in response to heat, suggesting that rice leaves are unable to regulate their transcriptome and proteome diversity in response to heat stress. ",
"Alternativ... | https://doi.org/10.3390/plants10081647 | Model Organisms | GENE REGULATION | 10.3390/plants10081647 | 2,021 | 17 | 1 | Plants | true |
How conserved is the regulation of alternative splicing in circadian clock orthologues from barley and Arabidopsis in response to cold? | GENE REGULATION - ALTERNATIVE SPLICING | [
"Hordeum vulgare",
"Arabidopsis thaliana"
] | [
"The regulation of alternative splicing in circadian clock orthologues shows mostly conserved mechanisms and rarely species-specific divergences between barley and Arabidopsis in response to cold. For example, GI, PRR7 and TOC1 orthologues show conservation of alternative splicing events and behaviour in response t... | https://doi.org/10.1371/journal.pone.0168028 | Model Organisms | GENE REGULATION | 10.1371/journal.pone.0168028 | 2,016 | 27 | 2 | PLOS ONE | true |
What is the speed of alternative splicing response to cold stress in Arabidopsis leaves? | GENE REGULATION - ALTERNATIVE SPLICING | [
"Arabidopsis thaliana"
] | [
"The alternative splicing response to cold stress in Arabidopsis leaves is rapid. More than half of the genes known to undergo alternative splicing in response to cold show differential gene expression within 9 hours of cold stress. Additionally, over half of the cold-induced isoform switches occurred 6 hours befor... | https://doi.org/10.1105/tpc.18.00177 | Model Organisms | GENE REGULATION | 10.1105/tpc.18.00177 | 2,018 | 242 | 1 | The Plant Cell | true |
Which is the most comprehensive Reference Transcript Dataset for Arabidopsis thaliana to date? | GENOME AND GENOMICS | [
"Arabidopsis thaliana"
] | [
"AtRTD3 is the most comprehensive and accurate Arabidopsis transcriptome as of 2024. It was constructed from sequencing RNAs from a diverse set of tissues, environmental conditions, and mutants, ensuring transcript diversity. Robust computational methods were used to ensure accuracy of transcript sequences. ",
"T... | https://doi.org/10.1186/s13059-022-02711-0 | Model Organisms | GENOME AND GENOMICS | 10.1186/s13059-022-02711-0 | 2,022 | 56 | 0 | Genome Biology | true |
Why is the AtRTD2-QUASI not the most appropriate Reference Transcript Dataset for quantifying Arabidopsis thaliana transcripts with bona fide alternative transcription start site and/or polyadenylation? | GENOME AND GENOMICS | [
"Arabidopsis thaliana"
] | [
"AtRTD2-QUASI is unable to properly quantify the expression for the majority of genes because it is built on the assumption that much of the variation in 5’ and 3’ UTR length of transcripts is likely due to transcripts lacking regulatory regions (i.e., not being full-length). Accordingly, modifying the transcripts ... | https://doi.org/10.1093/nar/gkx267 | Model Organisms | GENOME AND GENOMICS | 10.1093/nar/gkx267 | 2,017 | 234 | 1 | Nucleic Acids Research | true |
How UV-B radiation impacts in the UVR8 photoreceptor protein structure in Arabidopsis thaliana? | ENVIRONMENT - ABIOTIC STRESS | [
"Arabidopsis thaliana"
] | [
"After UV-B radiation, UVR8 is activated via a structural change from a monomer to dimer. The aminoacids tryptophans Trp- 285 and Trp-233 act as chromophores which absorb UV-B leading to conformational changes generating dimer interactions. ",
"After UV-B radiation, UVR8 is activated via a structural change from ... | https://doi.org/10.1146/annurev-arplant-050718-095946 | Model Organisms | ENVIRONMENT | 10.1146/annurev-arplant-050718-095946 | 2,021 | 108 | 2 | Annual Review of Plant Biology | true |
How is the E3 ubiquitin ligase COP1 involved in UV-B perception and signalling in Arabidopsis thaliana? | ENVIRONMENT - ABIOTIC STRESS | [
"Arabidopsis thaliana"
] | [
"COP1 forms an E3 ubiquitin ligase complex together with SPA proteins, and this complex represses photomorphogenesis in the dark by directing promoting factors, such as the UV-B photoreceptor UVR8, to be degraded. After UV-B radiation, the TF HY5 interacts with COP1. This interaction sequesters COP1 from the E3 ubi... | https://doi.org/10.1007/s44154-022-00076-9 | Model Organisms | ENVIRONMENT | 10.1007/s44154-022-00076-9 | 2,022 | 44 | 1 | Stress Biology | true |
Which proteins facilitate UVR8 redimerization in Arabidopsis thaliana? | ENVIRONMENT - ABIOTIC STRESS | [
"Arabidopsis thaliana"
] | [
"The proteins are WD40-repeat RUP1 and RUP2 proteins and their transcripts are UV-B inducible in a UVR8 dependent manner. These proteins interact with both UVR8 homodimer and monomers, although they have a stronger affinity for active UVR8 monomers. RUP1 and RUP2 facilitate redimerization of UVR8 in vivo within 2 h... | https://doi.org/10.1146/annurev-arplant-050718-095946 | Model Organisms | ENVIRONMENT | 10.1146/annurev-arplant-050718-095946 | 2,021 | 108 | 0 | Annual Review of Plant Biology | true |
Which is the subcellular localization and activity of UVR8 in Arabidopsis thaliana? | ENVIRONMENT - ABIOTIC STRESS | [
"Arabidopsis thaliana"
] | [
"UVR8 localizes both in the cytosol and the nucleus. Upon UV-B exposure, UVR8 monomers accumulate in the nucleus, although it does not have a nuclear localization signal (NLS). Intriguingly, COP1 is required for UVR8 nuclear accumulation, and it includes both an NLS and a nuclear export signal. Alternatively, UVR8 ... | https://doi.org/10.1146/annurev-arplant-050718-095946 | Model Organisms | ENVIRONMENT | 10.1146/annurev-arplant-050718-095946 | 2,021 | 108 | 0 | Annual Review of Plant Biology | true |
Which role plays the transcription factor HY5 in UV-B signaling in Arabidopsis thaliana? | ENVIRONMENT - ABIOTIC STRESS | [
"Arabidopsis thaliana"
] | [
"HY5 is a positive regulator of photomorphogenesis, and it directly binds to DNA to regulate expression of numerous genes. HY5 and its homolog HYH, show partially overlapping function in signalling pathways downstream of several photoreceptors; however, HY5 plays the major role under all light conditions. HY5 is al... | https://doi.org/10.1146/annurev-arplant-050718-095946 | Model Organisms | ENVIRONMENT | 10.1146/annurev-arplant-050718-095946 | 2,021 | 108 | 0 | Annual Review of Plant Biology | true |
In the framework of alternative splicing, what is an exitron? | GENE REGULATION - ALTERNATIVE SPLICING | [
"non-specific"
] | [
"It is a region of an RNA that can be recognized by the spliceosome as an intron but, in opposition to other introns (canonical introns), exitrons have coding capacities and other features that are common for exons. ",
"An exitron is a sequence of DNA that is both an intron and an exon, meaning it has characteris... | 10.1101/gr.186585.114 | Non-specific | GENE REGULATION | 10.1101/gr.186585.114 | 2,015 | 137 | 0 | Genome Research | true |
Considering gene expression in eukaryotes, the definition of the term exitron brings to debate more basic questions related to splicing and alternative splicing, in this sense, what is an intron? | GENE REGULATION - ALTERNATIVE SPLICING | [
"non-specific"
] | [
"An intron is a region of an RNA molecule that can be recognized by the spliceosome and spliced out or excised from the RNA molecule forming a lariat. ",
"Is an internal non-coding region of an RNA.",
"It is a segment of a DNA or RNA molecule which does not code for proteins and interrupts the sequence of genes... | non-specific | Non-specific | GENE REGULATION | null | null | null | 0 | null | true |
Considering the kinetic model that explains the coupling between alternative splicing regulation and RNA polymerase II elongation, a "slower" elongation would favor or not the inclusion of a cassette exon? | GENE REGULATION - ALTERNATIVE SPLICING | [
"non-specific"
] | [
"Slower elongation rates of RNA polymerase II are linked to higher exclusion of exon cassettes. ",
"In general a slower elongation rate would favor the inclusion of alternative exons with weak splice sites in their borders. However, there are specific cases in which there is a competition between splice site sele... | https://doi.org/10.1016/j.molcel.2014.03.044 | Non-specific | GENE REGULATION | 10.1016/j.molcel.2014.03.044 | 2,014 | 199 | 1 | Molecular Cell | true |
What is the most frequent alternative splicing event in plants? | GENE REGULATION - ALTERNATIVE SPLICING | [
"non-specific"
] | [
"In general, the most conspicuous splicing event in plants is intron retention. ",
"Exitron splicing is the most frequent event in plants.",
"Exon skipping. "
] | https://doi.org/10.1016/j.tplants.2019.02.006 | Non-specific | GENE REGULATION | 10.1016/j.tplants.2019.02.006 | 2,019 | 112 | 0 | Trends in Plant Science | true |
There are key differences between plants and animals, also in alternative splicing regulation. In animals the main outcome of alternative splicing of coding genes is the production of proteins with different functions. Would you say that in plants the scenario is the same? | GENE REGULATION - ALTERNATIVE SPLICING | [
"non-specific"
] | [
"There are no differences in this sense, the main outcome of alternative splicing is the generation of different isoforms that can be translated to different proteins.",
"The alternative splicing process generates different proteins, in plants as well as in animals.",
"In plants intron retention is the most com... | https://doi.org/10.1016/j.tplants.2019.02.006 | Non-specific | GENE REGULATION | 10.1016/j.tplants.2019.02.006 | 2,019 | 112 | 2 | Trends in Plant Science | true |
How are GRF transcription factors regulated, and how do they control leaf growth in Arabidopsis thaliana? | GROWTH AND DEVELOPMENT | [
"Arabidopsis thaliana"
] | [
"GRFs are transcriptionally regulated by GIF co-regulators and microRNA miR396 through the transcriptional gene silencing pathway. GRFs control leaf longevity and senescence. An increase in microRNA miR396 reduces GRF expression, inducing senescence and decreasing organ size.",
"GRFs are post-transcriptionally re... | 10.1242/dev.043067 | Model Organisms | GROWTH AND DEVELOPMENT | 10.1242/dev.043067 | 2,010 | 443 | 2 | Development | true |
How is the transcription factor ARF2 involved in the control of cell number in Arabidopsis leaves? | GROWTH AND DEVELOPMENT | [
"Arabidopsis thaliana"
] | [
"arf2 mutants have larger leaves with more cells. ARF2 directly activates GRF5, a transcription factor that is not regulated by microRNA miR396. Overexpression of GRF5 in arf2 mutants restores the cell number in leaves to wild-type levels.",
"arf2 mutants have larger leaves with more cells. ARF2 directly represse... | 10.1093/plphys/kiab014 | Model Organisms | GROWTH AND DEVELOPMENT | 10.1093/plphys/kiab014 | 2,021 | 30 | 1 | Plant Physiology | true |
What is the function of miR396 in Arabidopsis roots? | GROWTH AND DEVELOPMENT | [
"Arabidopsis thaliana"
] | [
"miR396 has well-known functions in leaves, but its roles in other organs, such as roots, have not yet been studied in detail.",
"miR396 controls the transition from cell proliferation to cell differentiation. High levels of miR396 in roots trigger cell differentiation.",
"miR396 controls the transition from st... | 10.1105/tpc.15.00452 | Model Organisms | GROWTH AND DEVELOPMENT | 10.1105/tpc.15.00452 | 2,015 | 141 | 2 | The Plant Cell | true |
How are miRNA precursors recognized by the processing machinery in plant cells? | GENE REGULATION - PTGS | [
"non-specific"
] | [
"All plant miRNA precursors have a 15-nt stem below the miRNA/miRNA* duplex, which is recognized by the DCL1 complex to make the first cut and release a pre-miRNA. The DCL1 complex makes a second cut 21 nucleotides away from the first cut to release the mature microRNA.",
"Plant miRNA precursors can have differen... | 10.1093/nar/gky853 | Non-specific | GENE REGULATION | 10.1093/nar/gky853 | 2,018 | 15 | 1 | Nucleic Acids Research | true |
Do mismatches in the miRNA/miRNA* region of the precursor impact miRNA precursor processing in Arabidopsis Thaliana? | GENE REGULATION - PTGS | [
"Arabidopsis thaliana"
] | [
"No, the only important factor is the presence of an overall double-stranded RNA region in the precursor.",
"Yes, the number and position of mismatches in the miRNA/miRNA* duplex determine whether the miRNA translationally inhibits its target or guides target cleavage.",
"Yes, mismatches can reduce the processi... | 10.1093/nar/gkae458 | Model Organisms | GENE REGULATION | 10.1093/nar/gkae458 | 2,024 | 4 | 2 | Nucleic Acids Research | true |
In Arabidopsis, the long non-coding RNA, SVALKA, represses CBF1 via which molecular mechanism? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"Transcriptional collision",
"Transcriptional regulation",
"Epigenetic regulation"
] | 10.1038/s41467-018-07010-6 | Model Organisms | GENE REGULATION | 10.1038/s41467-018-07010-6 | 2,018 | 177 | 0 | Nature Communications | true |
What is the hallmark of RNAPII collision in Arabidopsis? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"RNAPII stalling on both DNA strands detected by plaNET-seq",
"Prematurely terminated mRNA detected by RNA-seq",
"Increased occurrence of transcription end sites detected by Direct RNA-seq"
] | 10.1038/s41467-018-07010-6 | Model Organisms | GENE REGULATION | 10.1038/s41467-018-07010-6 | 2,018 | 177 | 0 | Nature Communications | true |
Why cannot two RNAPII complexes pass each other when transcribing on opposite DNA strands? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"DNA looping",
"Formation of R-loops",
"Steric hindrance"
] | 10.1016/j.molcel.2012.08.027 | Model Organisms | GENE REGULATION | 10.1016/j.molcel.2012.08.027 | 2,012 | 151 | 2 | Molecular Cell | true |
What is the prerequisite for transcriptional collision? | GENE REGULATION - TRANSCRIPTION | [
"non-specific"
] | [
"Phosphorylation of serine 2 of the NRPB1 CTD-tail",
"Simultaneous transcription of both DNA strands in opposite direction ",
"Increased RNAPII stalling on one DNA strand"
] | 10.1038/s41467-018-07010-6 | Non-specific | GENE REGULATION | 10.1038/s41467-018-07010-6 | 2,018 | 177 | 1 | Nature Communications | true |
Where is the main stalling site in the 5'-end of genes in Arabidopsis? | GENE REGULATION - TRANSCRIPTION | [
"Arabidopsis thaliana"
] | [
"The +1 nucleosome",
"The transcription start site",
"100 bp downstream of the transcription start site"
] | 10.1093/nar/gkz1189 | Model Organisms | GENE REGULATION | 10.1093/nar/gkz1189 | 2,019 | 91 | 0 | Nucleic Acids Research | true |
End of preview. Expand
in Data Studio
Dataset Card for MoBiPlant
Dataset Summary
MoBiPlant is a multiple-choice question-answering dataset curated by plant molecular biologists worldwide. It comprises two merged versions:
- Expert MoBiPlant: 565 expert-level questions authored by leading researchers.
- Synthetic MoBiPlant: 1,075 questions generated by large language models from papers in top plant science journals.
Each example consists of a question about plant molecular biology, a set of answer options, and the index of the correct answer. This dataset benchmarks MCQ-based knowledge in models within the plant molecular biology domain.
Dataset Details
- Name: MoBiPlant
- Version: v1.0
- GitHub: https://github.com/manoloFer10/mobiplant
- License: Creative Commons Attribution 4.0 International (CC BY 4.0)
- Release Date: 2025-06-09
Supported Tasks and Leaderboards
The primary task is:
- Multiple-Choice Question Answering: Given a question and a list of answer choices, predict the index of the correct option.
Leaderboard
Benchmark on Expert MoBiPlant (565 questions):
| Model | CoT Answer Accuracy (%) |
|---|---|
| LLaMA 3.1 405B | 77.6 |
| GPT-4o | 81.2 |
| o1-mini | 81.1 |
| deepseek v3 | 84.3 |
| deepseek-r1 | 86.4 |
| Claude 3.5 Sonnet | 88.1 |
| Gemini 1.5 Pro | 76.8 |
For full results on both versions, see the associated paper.
Languages
- Language: English
Dataset Structure
Versions:
- Expert: 565 expert-authored questions.
- Synthetic: 1,075 LLM-generated questions.
Splits:
- The
trainsplit contains all examples (1,640 total). To access each version, see Usage.
- The
Number of Examples:
- 1,640 total examples across expert and synthetic sets.
Data Fields
Each entry in the train split contains:
| Field | Type | Description |
|---|---|---|
question |
string |
The MCQ question text. |
options |
list[string] |
A list of possible answer strings. |
answer |
int |
Index of the correct option in options (0-based). |
area |
string |
General research area (e.g., GENE REGULATION - TRANSLATION). |
normalized_area |
string |
Normalized research area category (e.g., GENE REGULATION). |
plant_species |
list[string] |
Original plant species labels (e.g., ["Arabidopsis thaliana", "Zea mays"]). |
normalized_plant_species |
string |
Normalized plant species label (e.g., Non-specific). |
doi |
string |
DOI of the primary source publication. |
source |
string |
URL or citation of the source article. |
source_journal |
string |
Journal of publication of the source article. |
Year |
int |
Publication year of the source. |
Citations |
int |
Number of citations the source article has received. |
is_expert |
bool |
True if the example belongs to the Expert MoBiPlant subset; False otherwise. |
Usage
from datasets import load_dataset
# Load from HF
mobiplant = load_dataset("manufernandezbur/MoBiPlant")['train']
# Filter out expert and synthetic versions (optional)
expert_mobiplant = mobiplant.filter(lambda question: question['is_expert'])
synth_mobiplant = mobiplant.filter(lambda question: not question['is_expert'])
# Example iteration
for example in expert_mobiplant:
question = example["question"]
options = example["options"]
label = example["answer"]
print(f'Question: {question}')
print('Options: ','\n'.join([ chr(65+i) + opt for i,opt in enumerate(options)]))
print('Correct Answer: ', options[label])
Citation
@article {Fernandez Burda2025.08.31.672925,
author = {Fernandez Burda, Manuel and Ferrero, Lucia and Gaggion, Nicol{\'a}s and Fonouni-Farde, Camille and The MoBiPlant Consortium and Crespi, Mart{\'\i}n and Ariel, Federico and Ferrante, Enzo},
title = {What Large Language Models Know About Plant Molecular Biology},
elocation-id = {2025.08.31.672925},
year = {2025},
doi = {10.1101/2025.08.31.672925},
publisher = {Cold Spring Harbor Laboratory},
abstract = {Large language models (LLMs) are rapidly permeating scientific research, yet their capabilities in plant molecular biology remain largely uncharacterized. Here, we present MoBiPlant, the first comprehensive benchmark for evaluating LLMs in this domain, developed by a consortium of 112 plant scientists across 19 countries. MoBiPlant comprises 565 expert-curated multiple-choice questions and 1,075 synthetically generated questions, spanning core topics from gene regulation to plant-environment interactions. We benchmarked seven leading chat-based LLMs using both automated scoring and human evaluation of open-ended answers. Models performed well on multiple-choice tasks (exceeding 75% accuracy), although most of them exhibited a consistent bias towards option A. In contrast, expert reviews exposed persistent limitations, including factual misalignment, hallucinations, and low self-awareness. Critically, we found that model performance strongly correlated with the citation frequency of source literature, suggesting that LLMs do not simply encode plant biology knowledge uniformly, but are instead shaped by the visibility and frequency of information in their training corpora. This understanding is key to guiding both the development of next-generation models and the informed use of current tools in the everyday work of plant researchers. MoBiPlant is publicly available online in this link.Competing Interest StatementThe authors have declared no competing interest.},
URL = {https://www.biorxiv.org/content/early/2025/09/04/2025.08.31.672925},
eprint = {https://www.biorxiv.org/content/early/2025/09/04/2025.08.31.672925.full.pdf},
journal = {bioRxiv}
}
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